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Evidence Guide: MSL974035 - Prepare tissue or cell cultures

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL974035 - Prepare tissue or cell cultures

What evidence can you provide to prove your understanding of each of the following citeria?

Work safely according to the legal and regulatory framework

  1. Identify hazards and workplace controls associated with the sample, preparation methods, reagents and equipment
  2. Select, fit and use personal protective equipment (PPE)
  3. Address hazards and incidents as they arise in accordance with workplace procedures
  4. Maintain a chain of custody, traceable to the worker, for all cells and tissues
Identify hazards and workplace controls associated with the sample, preparation methods, reagents and equipment

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select, fit and use personal protective equipment (PPE)

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Address hazards and incidents as they arise in accordance with workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain a chain of custody, traceable to the worker, for all cells and tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare and test cell or tissue culture media

  1. Select and confirm media specifications and processes and methods
  2. Prepare culture media to suit the application
  3. Sterilise culture media and check for sterility
  4. Perform quality control checks to ensure that culture media is fit for purpose
  5. Store culture media in accordance with specifications
Select and confirm media specifications and processes and methods

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare culture media to suit the application

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Sterilise culture media and check for sterility

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform quality control checks to ensure that culture media is fit for purpose

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Store culture media in accordance with specifications

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Use tissue or cell culture preparation techniques

  1. Select tissue or cell sample to optimise growth and prepare it for culture
  2. Add specified growth agents and/or nutrients
  3. Inoculate culture medium using aseptic techniques
Select tissue or cell sample to optimise growth and prepare it for culture

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Add specified growth agents and/or nutrients

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inoculate culture medium using aseptic techniques

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Monitor tissue or cell culture

  1. Incubate culture in specified conditions
  2. Monitor growth of culture and record appearance and characteristics
  3. Identify expected cell types and recognise normal and abnormal cells
  4. Report presence or absence of contamination
  5. Subculture the culture to continue the cell line
  6. Dispose of biohazardous and other laboratory waste safely
  7. Maintain accurate records of batches of media
  8. Complete records of tissue cultures so that they are retrievable, legible and accurate
  9. Complete records in accordance with workplace and legal requirements
Incubate culture in specified conditions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Monitor growth of culture and record appearance and characteristics

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify expected cell types and recognise normal and abnormal cells

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Report presence or absence of contamination

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Subculture the culture to continue the cell line

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Dispose of biohazardous and other laboratory waste safely

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain accurate records of batches of media

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Complete records of tissue cultures so that they are retrievable, legible and accurate

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Complete records in accordance with workplace and legal requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge